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Light-field Microscopy with a Consumer Light-field Camera

机译:使用消费者光场相机的光场显微镜

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摘要

We explore the use of inexpensive consumer light- field camera technology forthe purpose of light-field mi- croscopy. Our experiments are based on the Lytro(first gen- eration) camera. Unfortunately, the optical systems of the Lytroand those of microscopes are not compatible, lead- ing to a loss of light-fieldinformation due to angular and spatial vignetting when directly recordingmicroscopic pic- tures. We therefore consider an adaptation of the Lytro op-tical system. We demonstrate that using the Lytro directly as an oc- ularreplacement, leads to unacceptable spatial vignetting. However, we also found asetting that allows the use of the Lytro camera in a virtual imaging mode whichprevents the information loss to a large extent. We analyze the new vir- tualimaging mode and use it in two different setups for im- plementing light-fieldmicroscopy using a Lytro camera. As a practical result, we show that the cameracan be used for low magnification work, as e.g. common in quality control,surface characterization, etc. We achieve a maximum spa- tial resolution ofabout 6.25{\mu}m, albeit at a limited SNR for the side views.
机译:我们探索将廉价的消费者光场相机技术用于光场显微检查的目的。我们的实验基于Lytro(第一代)相机。不幸的是,Lytro的光学系统和显微镜的光学系统不兼容,当直接记录显微照片时,由于角度和空间渐晕而导致光场信息的丢失。因此,我们考虑使用Lytro光学系统。我们证明了将Lytro直接用作耳廓置换会导致不可接受的空间渐晕。但是,我们还发现了一种设置,允许在虚拟成像模式下使用Lytro相机,从而在很大程度上防止了信息丢失。我们分析了新的虚拟成像模式,并在两种不同的设置中使用它,以使用Lytro相机实施光场显微镜检查。实际结果表明,该相机可用于低倍放大工作,例如在侧视图中,尽管信噪比有限,但我们实现了约6.25 {μm]的最大空间分辨率。

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